A Self‐Quenching System Based on Bis‐Naphthalimide: A Dual Two‐Photon‐Channel GSH Fluorescent Probe. Issue 13 (24th April 2017)

• Record Type: Journal Article
• Authors: Shen, Wei; Ge, Jingyan; He, Siyang; Zhang, Ruoyu; Zhao, Chengyan; Fan, Yong; Yu, Shian; Liu, Bin; Zhu, Qing
• Is Part Of: Chemistry, an Asian journal. Volume 12:Issue 13(2017)
• Page Start: 1532
• Page End: 1537
• Publication Date: 2017-04-24
• Subjects: aggregation-caused quenching -- fluorescent probes -- FRET -- naphthalimides -- two-photon probes; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201700340
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: A single fluorescent signal output often does not satisfy the detection accuracy requirements for bioactive compounds in biological systems. It is of great interest to develop a dual‐channel turn‐on fluorescence sensing system for self‐validated detection. Herein, we report a self‐quenching nanoparticle composed of dual two‐photon dyes of naphthalimides, displaying almost no fluorescence at both channels, owing to aggregation‐caused quenching (ACQ). A dual two‐photon‐channel fluorescent probe for GSH was further developed by inserting a disulfide bond between two naphthalimides. Upon treatment with GSH, the disulfide bond was cleaved, and two napthalimide fluorophores were separated, leading to turn‐on fluorescence at both channels. This dual‐two‐photon‐channel design strategy can be easily extended to other analytes, simply by changing the linker molecules, which creates new opportunities to accurately monitor various biological processes in living cells. Abstract : Photon cannons : A dual two‐photon‐channel probe for GSH detection was developed using a self‐quenching system based on fluorophores of 1, 8‐naphthalimides.