Comparative analysis of transcriptomic responses to repeated-dose exposure to 2-MCPD and 3-MCPD in rat kidney, liver and testis. (August 2017)

• Record Type: Journal Article
• Authors: Buhrke, Thorsten; Schultrich, Katharina; Braeuning, Albert; Lampen, Alfonso
• Is Part Of: Food and chemical toxicology. Volume 106:Part A(2017)
• Page Start: 36
• Page End: 46
• Publication Date: 2017-08
• Subjects: 3-Chloro-1, 2-propanediol -- 2-Chloro-1, 3-propanediol -- Toxicogenomics -- Food contaminant -- Oral exposure -- Nephrotoxicity; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.05.028
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: 3-Chloro-1, 2-propanediol (3-MCPD) and its isomer 2-chloro-1, 3-propanediol (2-MCPD) are heat-induced food contaminants present in oil- and fat-containing foodstuff. Kidney and testes are among the main target organs of 3-MCPD. Almost no data on 2-MCPD toxicity are available. Here, transcriptomic responses following repeated-dose exposure of rats to non-toxic doses of 10 mg/kg body weight per day 2-MCPD or 3-MCPD for 28 days were characterized by microarray analysis of kidney, liver, and testes. 3-MCPD exerted more pronounced effects than 2-MCPD in all organs. The limited overlap between the datasets indicates that 2-MCPD and 3-MCPD do not share the same molecular mechanisms of toxicity. By combining transcriptomic data with datasets on proteomic regulation by 3-MCPD, a comprehensive view on 3-MCPD-induced regulation of glucose utilization and oxidative stress response was developed. Bioinformatic analyses revealed that Nrf2 (nuclear factor (erythroid-derived 2)-like 2) signaling is likely to be involved in mediating the oxidative stress response to 3-MCPD. In summary, this study for the first time presents data on alterations in global gene expression by two important food contaminants, 2-MCPD and 3-MCPD. Data demonstrate profound differences between the effects of the two compounds and substantially broaden our knowledge on molecular details of 3-MCPD-induced disturbance of glucose utilization and redox balance. Highlights: Transcriptomic signatures induced by 3-MCPD and 2-MCPD are presented. A global view of regulation was obtained by combination with proteomic data. Data suggest different mechanisms of toxicity by 2-MCPD and 3-MCPD. 3-MCPD induces alterations of glucose utilization. 3-MCPD leads to a pronounced oxidative stress response in kidney and testes.