Astaxanthin from shrimp efficiently modulates oxidative stress and allied cell death progression in MCF-7 cells treated synergistically with β-carotene and lutein from greens. (August 2017)

• Record Type: Journal Article
• Authors: Sowmya, Poorigali Raghavendra-Rao; Arathi, Bangalore Prabhashankar; Vijay, Kariyappa; Baskaran, Vallikannan; Lakshminarayana, Rangaswamy
• Is Part Of: Food and chemical toxicology. Volume 106:Part A(2017)
• Page Start: 58
• Page End: 69
• Publication Date: 2017-08
• Subjects: Shrimp astaxanthin -- β-Carotene -- Lutein -- Synergism -- Cytotoxicity -- MCF-7 cells proliferation; AST Astaxanthin -- BC β-Carotene -- BHT Butylatedhydroxytoluene -- CM Carotenoids mixture -- DMEM Dulbecco's modified eagle's medium -- DMSO Dimethyl sulfoxide -- DTNB 5, 5-dithiobis (2-nitrobenzoic acid) -- FBS Fetal bovine serum -- GR Glutathione reductase -- L Lutein -- MDA Malondialdehyde -- MTT 3-[4, 5-dimethylthiazol-2-yl]-2, 5- diphenyltetrazolium bromide -- NADPH Nicotinamide adenine dinucleotide phosphate oxidase -- SSCE Saponified shrimp carotenoid extract -- THF Tetrahydrofuran -- TMP Tetramethoxypropane; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.05.024
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: This study investigated the synergistic efficacy of keto-carotenoid astaxanthin (AST, from shrimp) plus hydrocarbon (β-carotene, BC) and hydroxyl (lutein, L) carotenoids (from greens) on molecular events in MCF-7 cells. MCF-7 cells were treated with either of carotenoid (20 μM, AST or BC or L) separately or the mixture of them (an equimolar concentration of carotenoids mixture, CM) or saponified carotenoid extract from shrimp (SSCE) for 48 h and analyzed cellular uptake, cytotoxicity, and apoptosis. The IC50 and combination-index values of AST co-treatment with a lower concentration of BC and L (5 μM) exhibited enhanced cytotoxicity and oxidative stress as compared with individual carotenoids or SSCE. Further, higher cellular uptake/accumulation of AST along with BC and L found to synergistically induce apoptosis through modulation of cyclin D1, p53, Bax and Bcl-2 expressions by arresting cell cycle at G0/G1 phase. Further, CM or SSCE treatments are unlikely to affect proliferation of normal breast epithelial cells (MCF-10A). The results of selective killing of MCF-7 cells demonstrated a greater insight on the synergistic effect of shrimp AST plus BC and L. It is concluded that consumption of shrimp along with green leafy vegetables helps in combating cancer chemoprevention. Highlights: Cytotoxic effect of astaxanthin with β-carotene and lutein is superior as compared to individual carotenoid in MCF-7 cells. Carotenoids mixture/shrimp carotenoids modulate apoptogenic properties by increasing ROS with depleted GSH and higher MDA levels. Carotenoid mixture from shrimp and green leafy vegetables is advisable to achieve maximum health benefits.