Antitumor activity of the pachymic acid in nasopharyngeal carcinoma cells. (4th May 2017)

• Record Type: Journal Article
• Authors: Zhang, Yan-Hua; Zhang, Yong; Li, Xiu-Ying; Feng, Xu-Dong; Jian, Wei; Li, Rong-Qing
• Is Part Of: Ultrastructural pathology. Volume 41:Number 3(2017:Jun.)
• Page Start: 245
• Page End: 251
• Publication Date: 2017-05-04
• Subjects: Pachymic acid -- NPC -- proliferative -- apoptosis -- DNA damage -- TEM; Pathology, Cellular -- Periodicals; Ultrastructure (Biology) -- Periodicals; Diagnosis, Electron microscopic -- Periodicals; Microscopy, Electron; Pathology; 616.07582
• DOI: 10.1080/01913123.2017.1296522
• Languages: English
• ISSNs: 0191-3123

Abstract

ABSTRACT: Objective : To investigate the antitumour efficacy of pachymic acid (PA), which is a fungal extract component, on nasopharyngeal carcinoma (NPC) cells CNE-1, CNE-2.Methods : We have chosen NPC cell line CNE-2 for the study, and the cells were treated with PA before the detection. CCK-8 assay was used to detect the proliferative ability, and Annexin V-PI double staining was used for the detection of apoptosis rate; and the nucleus damage was detected by transmission electron microscope, the protein expression of the DNA damage pathway were detected by Western blot.Results : PA can significantly inhibited proliferation of CNE-1, CNE-2 cells. The proportion of apoptotic cells of all cell lines gradually increased in a dose-dependent manner induced by PA, P < 0.05. Meanwhile, the nucleus could be caused morphological changes and the expression of DNA damage-related proteins was upregulated by PA in CNE-2.Conclusions : PA can significantly inhibit cell proliferation and increase the apoptosis rates and may induce the apoptosis of the human NPC cells.