An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers. Issue 13 (2nd June 2017)

• Record Type: Journal Article
• Authors: Hu, Binfeng; Li, Juanjuan; Mou, Lei; Liu, Yong; Deng, Jinqi; Qian, Wei; Sun, Jiashu; Cha, Ruitao; Jiang, Xingyu
• Is Part Of: Lab on a chip. Volume 17:Issue 13(2017)
• Page Start: 2225
• Page End: 2234
• Publication Date: 2017-06-02
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c7lc00249a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : An automated and portable microfluidic chemiluminescence immunoassay is developed for quantitative detection of biomarkers in serum samples. Abstract : Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.