Optical mapping design for murine atrial electrophysiology. Issue 5 (3rd September 2017)

• Record Type: Journal Article
• Authors: Yu, T.Y.; Dehghani, H.; Brain, K.; Syeda, F.; Holmes, A. P.; Kirchhof, P.; Fabritz, L.
• Is Part Of: Computer methods in biomechanics and biomedical engineering. Volume 5:Issue 5(2017)
• Page Start: 368
• Page End: 376
• Publication Date: 2017-09-03
• Subjects: Optical mapping -- cardiovascular disease -- voltage imaging -- electrophysiology -- action potential duration; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Biomechanics -- Data processing -- Periodicals; Biomedical engineering -- Periodicals; 616.0757
• DOI: 10.1080/21681163.2015.1081079
• Languages: English
• ISSNs: 2168-1163

Abstract

Abstract: Optical mapping is an important tool for assessment of cardiac electrophysiology. We demonstrate a system for quantification and automatic measurement of electrophysiological parameters in isolated cardiac tissue. The system makes use of voltage sensitive fluorescent dyes. These shift in wavelength in response to millivolt changes in potential across cell membranes located on the left atrium. Automated analysis of the pixel-wise measurements yields information on action potential durations and isochronal maps allowing for high throughput of data analysis. The algorithms that we propose reliably describe activation sequences and allow for quantification of conduction velocities.