Exploring the effect of formulation parameters on the particle size of carboxymethyl chitosan nanoparticles prepared via reverse micellar crosslinking. (3rd April 2017)

• Record Type: Journal Article
• Authors: Sheikholeslami, Zeinab Sadat; Salimi-Kenari, Hamed; Imani, Mohammad; Atai, Mohammad; Nodehi, Azizollah
• Is Part Of: Journal of microencapsulation. Volume 34:Number 3(2017)
• Page Start: 270
• Page End: 279
• Publication Date: 2017-04-03
• Subjects: Carboxymethyl chitosan -- reverse micellar crosslinking -- nanoparticles -- experimental design; Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.1080/02652048.2017.1321047
• Languages: English
• ISSNs: 0265-2048

Abstract

Abstract: Carboxymethyl chitosan (CMCh), as a non-toxic, biocompatible and biodegradable semi-synthetic biopolymer with mucoadhesive properties, is widely investigated for targeting drug or gene delivery applications. Here, fabrication of CMCh nanoparticles will be reported using a reverse (w/o) micellar system to provide particles with tuneable mean sizes. Reverse microemulsions were prepared by dispersing aqueous solution of CMCh in n -hexane using sodium-bis(ethylhexyl)-sulfosuccinate (AOT) and glutaraldehyde (GA) as an emulsifier and a crosslinking agent, respectively. The obtained particles were perfectly spherical in the nanometric size range (40–140 nm) with a narrow size distribution according to the FE-SEM images. To determine the effective parameters on the mean nanosphere size, CMCh, AOT and GA concentrations were varied according to a full-factorial, three-level design-of-experiments. After evaluating the results, it was found that AOT and CMCh concentrations had a significant effect on the mean nanosphere size while GA concentration surprisingly showed non-significant, minor effects on the same response.