Antiplasmodial potential of extracts from two species of genus Blumea. (14th June 2013)

• Record Type: Journal Article
• Authors: Upadhyay, Harish C.; Sisodia, Brijesh S.; Verma, Ram K.; Darokar, Mahendra P.; Srivastava, Santosh K.
• Is Part Of: Pharmaceutical biology. Volume 51:Number 10(2013:Oct.)
• Page Start: 1326
• Page End: 1330
• Publication Date: 2013-06-14
• Subjects: Cytotoxicity -- fingerprinting -- HPLC -- selectivity index -- TCM; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.790453
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context : A number of Blumea (Asteraceae) species are being used in traditional Chinese and Indian folklore medicines to cure various diseases including cancer, fungal and bacterial infections. Objective : To evaluate the in vitro antiplasmodial potential and cytotoxicity of various extracts and fractions of B. membranacea DC and B. eriantha DC and high performance liquid chromatography (HPLC) chemical fingerprinting of their crude extracts. Materials and methods: The aerial parts and roots of B. membranacea and B. eriantha were extracted with ethanol and the extracts were successively partitioned with n -hexane, ethyl acetate and n -butanol, which were later evaluated for their in vitro antiplasmodial activity against Plasmodium falciparum NF-54 and in vitro cytotoxicities against non-cancerous Vero cell line. HPLC chemical fingerprinting was performed on extracts of B. membranacea and B. eriantha . Results : The n -hexane (MA1), ethyl acetate (MA2) fractions of aerial parts and n -butanol (MR3) fraction of roots of B. membranacea showed IC50 values of 17.4, 19.0 and 3.3 µg/mL respectively, while the n -hexane (EA1), ethyl acetate (EA2) fractions of aerial parts and ethyl acetate (ER2) fraction of roots of B. eriantha showed IC50 values of 25.0, 26.5 and 15.6 µg/mL, respectively, against P. falciparum NF-54. All these fractions were non-toxic to Vero cells. Discussion and conclusion : Both B. membranacea and B. eriantha possesses a high degree of selective antiplasmodial activity (selectivity index up to >60) and hence, may find their use in antimalarial phytopharmaceuticals as well as in discovery of a safer and novel antimalarial lead.