Low‐dose fixed‐target serial synchrotron crystallography. Issue 4 (1st April 2017)

• Record Type: Journal Article
• Authors: Owen, Robin L.; Axford, Danny; Sherrell, Darren A.; Kuo, Anling; Ernst, Oliver P.; Schulz, Eike C.; Miller, R. J. Dwayne; Mueller-Werkmeister, Henrike M.
• Is Part Of: Acta crystallographica. Volume 73:Issue 4(2017)
• Page Start: 373
• Page End: 378
• Publication Date: 2017-04-01
• Subjects: macromolecular crystallography -- serial synchrotron crystallography -- fixed target -- room‐temperature crystallography -- crystallography on a chip -- low dose; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798317002996
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : A time‐ and sample‐efficient approach for the serial collection of room‐temperature diffraction data using a fixed target at a synchrotron is demonstrated. Abstract : The development of serial crystallography has been driven by the sample requirements imposed by X‐ray free‐electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed‐target approach to high‐throughput serial sampling, it is demonstrated that high‐quality data can be collected from myoglobin crystals, allowing room‐temperature, low‐dose structure determination. The combination of fixed‐target arrays and a fast, accurate translation system allows high‐throughput serial data collection at high hit rates and with low sample consumption.