A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase. Issue 51 (12th June 2017)

• Record Type: Journal Article
• Authors: Ma, Fei; Liu, Wen-jing; Tang, Bo; Zhang, Chun-yang
• Is Part Of: Chemical communications. Volume 53:Issue 51(2017)
• Page Start: 6868
• Page End: 6871
• Publication Date: 2017-06-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc03736h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We develop a single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase with high sensitivity and good selectivity. Abstract : We develop a single quantum dot (QD)-based nanosensor for the signal-on detection of DNA methyltransferase (MTase). By integration of single-molecule counting with the QD-based fluorescence resonance energy transfer (FRET), the proposed nanosensor can sensitively detect DNA MTase with a detection limit of as low as 0.002 U mL −1, and it can be further applied for inhibitor screening and accurate detection of DNA MTase in complex biological samples.