Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent. Issue 24 (9th June 2017)

• Record Type: Journal Article
• Authors: Hou, Hai; Wang, Bo; Xie, Peng-Peng; Guo, Yun-Zhu; Li, Jin; Yin, Da-Chuan
• Is Part Of: CrystEngComm. Volume 19:Issue 24(2017)
• Page Start: 3237
• Page End: 3243
• Publication Date: 2017-06-09
• Subjects: Crystals -- Periodicals; Crystal growth -- Periodicals; Crystallography -- Periodicals; Cristaux -- Périodiques; Cristaux -- Croissance -- Périodiques; Cristallographie -- Périodiques; 548
• DOI: 10.1039/c7ce00664k
• Languages: English
• ISSNs: 1466-8033

Abstract

Abstract : We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". Abstract : We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.