A novel H2O2 biosensor based on three-dimensional micro/nano-biointerfaces. Issue 22 (19th May 2017)

• Record Type: Journal Article
• Authors: Wang, Qi; Hu, Sisheng; Yang, Tian; Ma, Shangshang; Liu, Yuhong; Ma, Chunxue; Wan, Mimi; Mao, Chun
• Is Part Of: Journal of materials chemistry. Volume 5:Issue 22(2017)
• Page Start: 4233
• Page End: 4238
• Publication Date: 2017-05-19
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c7tb00353f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A novel H2 O2 biosensor was first reported to directly grow living cells on three-dimensional micro/nano-biointerfaces to promote electrochemical performance. Abstract : To detect hydrogen peroxide (H2 O2 ) that is released in situ by cells is a challenge because of its high activity and short life time. Here, a new type of H2 O2 biosensor was reported based on three-dimensional (3D) micro/nano-biointerfaces as an amicable platform to promote cell adhesion, and which can directly grow living cells on manganese dioxide (MnO2 ) immobilized onto a novel hollow horn-like morphology polypyrrole (hPPy) film. The composite MnO2 /hPPy array films were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elemental mapping. Further, the results of a cell adhesion test indicated that the as-obtained 3D micro/nano-array films had good cell adhesion. This proposed sensing platform provided high electroactivity and excellent electron transport with a lower detection limit, a higher sensitivity and a short diffusion distance to reaction sites by electrochemical study. The new design strategy we proposed can expand the bioapplication of 3D micro/nano-biointerfaces that can guide cell fate and electrochemical detection in cell biology.