Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences. Issue 2 (16th November 2016)

• Record Type: Journal Article
• Authors: Raibaut, Laurent; Vasseur, William; Shimberg, Geoffrey D.; Saint-Pierre, Christine; Ravanat, Jean-Luc; Michel, Sarah L. J.; Sénèque, Olivier
• Is Part Of: Chemical science. Volume 8:Issue 2(2017)
• Page Start: 1658
• Page End: 1664
• Publication Date: 2016-11-16
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6sc04086a
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : We report the design of a luminescent sensor based upon the zinc finger protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3′-untranslated region of messenger RNA. Abstract : We report the design of a luminescent sensor based upon the zinc finger (ZF) protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3′-untranslated region of messenger RNA. This sensor is composed of the tandem ZF RNA binding domain of TIS11d functionalized with a luminescent Tb 3+ complex on one of the ZFs and a sensitizing antenna on the other. This work provides the proof of principle that an RNA binding protein can be re-engineered as an RNA sensor and, more generally, that tunable synthetic luminescent probes for biomolecules can be obtained by modifying biomolecule-binding domains.