Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay. (June 2017)

• Record Type: Journal Article
• Authors: Liu, Liyuan; Jiang, Luzhi; Yu, Yongxin; Xia, Xiaoming; Pan, Yingjie; Yan, Shuling; Wang, Yongjie
• Is Part Of: Molecular and cellular probes. Volume 33(2017)
• Page Start: 4
• Page End: 7
• Publication Date: 2017-06
• Subjects: Acute hepatopancreatic necrosis disease -- Vibrio owensii -- Recombinase polymerase amplification -- RPA -- Diagnosis; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2017.02.001
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii . The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis. Highlights: A rapid and sensitive AHPND-RPA assay was developed for the detection of the AHPND-causing agent of Vibrio owensii . The AHPND-RPA assay detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C. The AHPND-RPA assay showed 100% positive predictive value and negative predictive value for AHPND diagnosis.