Light transfer in agar immobilized microalgae cell cultures. (September 2017)

• Record Type: Journal Article
• Authors: Kandilian, Razmig; Jesus, Bruno; Legrand, Jack; Pilon, Laurent; Pruvost, Jérémy
• Is Part Of: Journal of quantitative spectroscopy & radiative transfer. Volume 198(2017)
• Page Start: 81
• Page End: 92
• Publication Date: 2017-09
• Subjects: Modeling -- Biofilm -- Radiation characteristics -- Immobilized microalgae -- Agar-entrapped -- Photobioreactors; Spectrum analysis -- Periodicals; Radiation -- Periodicals; Analyse spectrale -- Périodiques; Rayonnement -- Périodiques; Radiation; Spectrum analysis; Periodicals; 543.0858
• DOI: 10.1016/j.jqsrt.2017.04.027
• Languages: English
• ISSNs: 0022-4073

Abstract

Highlights: Light transfer through microalgae immobilized in agar films was modeled for the first time. Radiation characteristics of agar films and P. kessleri cells were measured separately. Model predictions of films transmittance and reflectance agreed well with measurements. Reflectance at 750 nm can be used for remote sensing of film biomass concentration. Optimal areal biomass concentration increased logarithmically with incident irradiance. Graphical abstract: Abstract: This paper experimentally and theoretically investigates light transfer in agar-immobilized cell cultures. Certain biotechnological applications such as production of metabolites secreted by photosynthetic microorganisms require cells to be immobilized in biopolymers to minimize contamination and to facilitate metabolite recovery. In such applications, light absorption by cells is one of the most important parameters affecting cell growth or metabolite productivity. Modeling light transfer therein can aid design and optimize immobilized-cell reactors. In this study, Parachlorella kessleri cells with areal biomass concentrations ranging from 0.36 to 16.9 g/m 2 were immobilized in 2.6 mm thick agar gels. The average absorption and scattering cross-sections as well as the scattering phase function of P. kessleri cells were measured. Then, the absorption and transport scattering coefficients of the agar gel were determined using an inverse method based on the modified two-flux approximation. The forward model was used to predict the normal-hemispherical transmittance and reflectance of the immobilized-cell films accounting for absorption and scattering by both microalgae and the agar gel. Good agreement was found between the measured and predicted normal-hemispherical transmittance and reflectance provided absorption and scattering by agar were taken into account. Moreover, good agreement was found between experimentally measured and predicted mean rate of photon absorption. Finally, optimal areal biomass concentration was determined to achieve complete absorption of the incident radiation.