DNA damage inhibits lateral root formation by up‐regulating cytokinin biosynthesis genes in Arabidopsis thaliana. (23rd September 2016)

• Record Type: Journal Article
• Authors: Davis, La Ode Muhammad Muchdar; Ogita, Nobuo; Inagaki, Soichi; Takahashi, Naoki; Umeda, Masaaki
• Is Part Of: Genes to cells. Volume 21:Number 11(2016)
• Page Start: 1195
• Page End: 1208
• Publication Date: 2016-09-23
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12436
• Languages: English
• ISSNs: 1356-9597

Abstract

Abstract : Lateral roots (LRs) are an important organ for water and nutrient uptake from soil. Thus, control of LR formation is crucial in the adaptation of plant growth to environmental conditions. However, the underlying mechanism controlling LR formation in response to external factors has remained largely unknown. Here, we found that LR formation was inhibited by DNA damage. Treatment with zeocin, which causes DNA double‐strand breaks, up‐regulated several DNA repair genes in the LR primordium (LRP) through the signaling pathway mediated by the transcription factor SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1). Cell division was severely inhibited in the LRP of zeocin‐treated sog1‐1 mutant, which in turn inhibited LR formation. This result suggests that SOG1‐mediated maintenance of genome integrity is crucial for proper cell division during LRP development. Furthermore, zeocin induced several cytokinin biosynthesis genes in a SOG1‐dependent manner, thereby activating cytokinin signaling in the LRP. LR formation was less inhibited by zeocin in mutants defective in cytokinin biosynthesis or signaling, suggesting that elevated cytokinin signaling is crucial for the inhibition of LR formation in response to DNA damage. We conclude that SOG1 regulates DNA repair and cytokinin signaling separately and plays a key role in controlling LR formation under genotoxic stress. Abstract : Lateral roots (LRs) are an important organ for water and nutrient uptake from soil. However, the underlying mechanism controlling LR formation in response to external factors has remained largely unknown. In this study, we revealed that SOG1 transcription factor regulates DNA repair and cytokinin signaling separately and plays a key role in controlling LR formation under genotoxic stress.