Characterization of OXA-48-like-producing Enterobacteriaceae isolated from river water in Algeria. (1st September 2017)

• Record Type: Journal Article
• Authors: Tafoukt, Rima; Touati, Abdelaziz; Leangapichart, Thongpan; Bakour, Sofiane; Rolain, Jean-Marc
• Is Part Of: Water research. Volume 120(2017)
• Page Start: 185
• Page End: 189
• Publication Date: 2017-09-01
• Subjects: Enterobacteriaceae -- OXA-48-like -- MLST -- Soummam river -- Algeria; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2017.04.073
• Languages: English
• ISSNs: 0043-1354

Abstract

Abstract: The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Isolates were subjected to antimicrobial susceptibility testing and the modified Carba NP test. Carbapenemase and extended-spectrum β-lactamase (ESBL) determinants were studied by PCR amplification and sequencing. The clonal relatedness between isolates was studied by Multilocus Sequence Typing (MLST) method. A total of 20 carbapenem-resistant Enterobacteriaceae strains were included in this study, identified as Escherichia coli (n = 12), Klebsiella pneumoniae (n = 3), Raoultella ornithinolytica (n = 3), Citrobacter freundii (n = 1) and Citrobacter braakii (n = 1). Carbapenemase genes identified in this study included bla OXA-48, observed in 17 isolates (9 E. coli, 3 K. pneumoniae, 3 R. ornithinolytica, 1 C. freundii and 1 C. braakii ), and bla OXA-244, a variant of bla OXA-48, was found in three E. coli isolates. MLST showed that 12 E. coli strains belonged to six different sequence types (ST559, ST38, ST212, ST3541, 1972 and ST2142), and we identified three different STs in K. pneumoniae isolates, including ST133, ST2055, and a new sequence type: ST2192. This study showed the presence of OXA-48-like-producing Enterobacteriaceae in water environments and highlighted the potential role of aquatic environments as reservoirs of clinically relevant antimicrobial-resistant bacteria, with the potential to spread throughout the community. Graphical abstract: Location of sampling sites and repartition of carbapenemase-producing Enterobacteriaceae isolated from the Soummam River, Bejaia (Algeria). Highlights: Carbapenemase-producing Enterobacteriaceae from water in Bejaia, Algeria. First report of bla OXA-48 producing Enterobacteriaceae from River water in Algeria. First report of bla OXA-244 gene in E. coli strains in Algeria. Detection of a new ST2192 in OXA-48-producing K. pneumoniae strain.