Nontoxic outer membrane vesicles efficiently increase the efficacy of an influenza vaccine in mice and ferrets. Issue 30 (27th June 2017)

• Record Type: Journal Article
• Authors: Shim, Sang-Mu; Song, Eun-Jung; Song, Daesub; Lee, Tae-Young; Kim, Doo-Jin; Nam, Jeong-Hyun; Gwin Jeong, Dae; Lee, Chong-Kil; Kim, Sang-Hyun; Kim, Jeong-Ki
• Is Part Of: Vaccine. Volume 35:Issue 30(2017)
• Page Start: 3741
• Page End: 3748
• Publication Date: 2017-06-27
• Subjects: fmOMV -- Adjuvant -- Influenza -- Pandemic -- T cell; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2017.05.053
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: The fmOMV–adjuvanted vaccine showed protective effect of the in animal models. The fmOMV with vaccine provided an efficient protection against virus challenges. The fmOMV–adjuvanted vaccine induced Th1-type immunity. fmOMV seems to be a promising candidate T cell adjuvant for the influenza vaccine. Abstract: In this study, we developed a further-modified outer membrane vesicle (fmOMV) from the Δ msbB/ΔpagP mutant of Escherichia coli transformed with the plasmid, pLpxF, in order to use it as an adjuvant for pandemic H1N1 (pH1N1) influenza vaccine. We evaluated the efficacy of the pH1N1 influenza vaccine containing the fmOMV in animal models as compared to the commercial adjuvants, alum or AddaVax TM . The fmOMV–adjuvanted pH1N1 influenza vaccine induced a significant increase in the humoral immunity; however, this effect was less than that of the AddaVax TM . The fmOMV–adjuvanted vaccine displayed pronounced an enhanced protective efficacy with increased T cell immune response and reduced the viral load in the lungs of the infected mice after challenging them with a lethal dose of the homologous virus. Moreover, it resulted in a significantly higher cross-protection against heterologous virus challenge than that of the pH1N1 vaccine with alum or with no adjuvants. In ferrets, the fmOMV–adjuvanted vaccine elicited a superior antibody response based on the HI titer and efficiently protected the animals from the lethal viral challenges. Taken together, the nontoxic fmOMV could be a promising adjuvant for inducing robust T cell priming into the pH1N1 vaccine and might be broadly applicable to the development of preventive measures against influenza virus infection.