Quantitative Assessment of Tumor Blood Flow Changes in a Murine Breast Cancer Model After Adriamycin Chemotherapy Using Contrast‐Enhanced Destruction‐Replenishment Sonography. (1st April 2013)

• Record Type: Journal Article
• Authors: Wang, Jian-Wei; Zheng, Wei; Chen, Yao; Cao, Long-Hui; Luo, Rong-Zhen; Li, An-Hua; Zhou, Jian-Hua
• Is Part Of: Journal of ultrasound in medicine. Volume 32:Number 4(2013)
• Page Start: 683
• Page End: 690
• Publication Date: 2013-04-01
• Subjects: chemotherapy -- contrast-enhanced sonography -- destruction-reperfusion -- tumor; Ultrasonics in medicine -- Periodicals; Ultrasonics; Ultrasonography; Ultrasonics in medicine; Electronic journals; Periodicals; Periodicals; 616.07543
• DOI: 10.7863/jum.2013.32.4.683
• Languages: English
• ISSNs: 0278-4297

Abstract

Abstract : Objectives: The purpose of the study was to detect tumor blood flow changes after chemotherapy with contrast‐enhanced destruction‐replenishment sonography. Methods: Twenty‐four MCF‐7 breast cancer–bearing nude mice were included in this study. Animals received either adriamycin or sterile saline and underwent contrast‐enhanced sonography before and after treatment using a destruction‐replenishment technique. A monoexponential function, y = A (1 − e −β t ), was used to fit the replenishment kinetics, where the plateau signal intensity A reflects the percent blood volume; the time constant β reflects the average speed of blood; and their product A *β reflects the nutrient blood flow. Tumor blood perfusion was compared to measurements of cell density and microvascular density. Results: Volumes of the treated tumors were significantly reduced after 7 days of adriamycin treatment compared with the control tumors ( P < .001). Before adriamycin administration, there was no significant difference in blood perfusion between the treated and control groups ( P > .05). Treatment with adriamycin resulted in a significant decrease in A, β, and A *β ( P <.001) compared with the control tumors. The tumor cell density and microvascular density estimated by pathologic slices were significantly lower in the treated tumors than in the control tumors ( P <.001). Conclusions: Quantification of tumor blood flow using contrast‐enhanced destruction‐replenishment sonography shows the potential to evaluate tumor responses to chemotherapy.