Integrated Biophysical and Biochemical Signals Augment Megakaryopoiesis and Thrombopoiesis in a Three‐Dimensional Rotary Culture System. (23rd December 2015) - Yang, Yiqing
Abstract : Platelet transfusion has been widely used in patients undergoing chemotherapy or radiotherapy; however, the shortage of the platelet supply limits the care of patients. A rotary cell culture system (RCCS) was evaluated to potentiate megakaryopoiesis and significantly improve the efficiency of platelet generation. The cost‐effective and highly controllable strategy and methodology represent an important step toward large‐scale platelet production for future biomedical and clinical applications. Abstract : Platelet transfusion has been widely used in patients undergoing chemotherapy or radiotherapy; however, the shortage of the platelet supply limits the care of patients. Although derivation of clinical‐scale platelets in vitro could provide a new source for transfusion, the devices and procedures for deriving scalable platelets for clinical applications have not been established. In the present study, we found that a rotary cell culture system (RCCS) can potentiate megakaryopoiesis and significantly improve the efficiency of platelet generation. When used with chemical compounds and growth factors identified via small‐scale screening, the RCCS improved platelet generation efficiency by as much as ∼3.7‐fold compared with static conditions. Shear force, simulated microgravity, and better diffusion of nutrients and oxygen from the RCCS, altogether, might account for the improved efficient platelet generation. The cost‐effective and highly controllable strategy and methodology represent an important step toward large‐scale platelet production for future biomedical and clinical applications. Significance: Platelet transfusion has been widely used in patients undergoing chemotherapy or radiotherapy; however, the shortage of platelet supply limits the care of patients. Thus, derivation of clinical‐scale platelets in vitro would provide a new source for transfusion. The present study evaluated a rotary suspension cell culture system that was able to potentiate megakaryopoiesis and significantly improved the efficiency of platelet generation. When used with chemical compounds and growth factors identified via small‐scale screening, the three‐dimensional system improved platelet generation efficiency compared with the static condition. The three‐dimensional device and the strategy developed in the present study should markedly improve the generation of large‐scale platelets for use in future biomedical and clinical settings.