Identification of a target protein of Hydra actinoporin-like toxin-1 (HALT-1) using GST affinity purification and SILAC-based quantitative proteomics. (July 2017)

• Record Type: Journal Article
• Authors: Ameirika, ; Sha, Hong Xi; Hwang, Jung Shan
• Is Part Of: Toxicon. Volume 133(2017)
• Page Start: 153
• Page End: 161
• Publication Date: 2017-07
• Subjects: Actinoporin -- Cytolysin -- Folate receptor alpha -- Pore-forming protein -- Hydra -- Protein-protein interactions; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2017.05.007
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Hydra actinoporin-like toxin-1 (HALT-1) is a 20.8 kDa pore-forming toxin isolated from Hydra magnipapillata . HALT-1 shares structural similarity with actinoporins, a family that is well known for its haemolytic and cytolytic activity. However, the precise pore-forming mechanism of HALT-1 remains an open question since little is known about the specific target binding for HALT-1. For this reason, a comprehensive proteomic analysis was performed using affinity purification and SILAC-based mass spectrometry to identify potential protein-protein interactions between mammalian HeLa cell surface proteins and HALT-1. A total of 4 mammalian proteins was identified, of which only folate receptor alpha was further verified by ELISA. Our preliminary results highlight an alternative-binding mode of HALT-1 to the human plasma membrane. This is the first evidence showing that HALT-1, an actinoporin-like protein, binds to a membrane protein, the folate receptor alpha. This study would advance our understanding of the molecular basis of toxicity of pore-forming toxins and provide new insights in the production of more potent inhibitors for the toxin-membrane receptor interactions. Highlights: Affinity of Hydra HALT-1 to sphingomyelin is very low and an alternative binding site may be involved. SILAC-based mass spectrometry was used to identify HALT-1 targeting membrane protein. Folate receptor alpha, a GPI-anchor protein interacts with HALT-1 in GST pull down assay. Direct binding of HALT-1 and folate receptor alpha was evaluated using ELISA.