Enhancing In Vivo Survival of Adipose‐Derived Stromal Cells Through Bcl‐2 Overexpression Using a Minicircle Vector. (9th August 2013)

• Record Type: Journal Article
• Authors: Hyun, Jeong; Grova, Monica; Nejadnik, Hossein; Lo, David; Morrison, Shane; Montoro, Daniel; Chung, Michael; Zimmermann, Andrew; Walmsley, Graham G.; Lee, Min; Daldrup-Link, Heike; Wan, Derrick C.; Longaker, Michael T.
• Is Part Of: Stem cells translational medicine. Volume 2:Number 9(2013)
• Page Start: 690
• Page End: 702
• Publication Date: 2013-08-09
• Subjects: Apoptosis -- Adult stem cells -- Cellular therapy -- Gene delivery systems in vivo or in vitro; Stem cells -- Periodicals; Regenerative medicine -- Periodicals; Periodicals; 616.0277405
• DOI: 10.5966/sctm.2013-0035
• Languages: English
• ISSNs: 2157-6564

Abstract

Abstract : For stem cell therapy to be effective, strategies are needed to prevent cell death and allow tissue formation. Using several strategies to overexpress the prosurvival protein Bcl‐2, this study demonstrated a decrease in the mediators of apoptosis in vitro and in vivo, an increased rate of survival within the wound environment, and an increase in tissue regeneration. Abstract : Tissue regeneration using progenitor cell‐based therapy has the potential to aid in the healing of a diverse range of pathologies, ranging from short‐gut syndrome to spinal cord lesions. However, there are numerous hurdles to be overcome prior to the widespread application of these cells in the clinical setting. One of the primary barriers to effective stem cell therapy is the hostile environment that progenitor cells encounter in the clinical injury wound setting. In order to promote cellular survival, stem cell differentiation, and participation in tissue regeneration, relevant cells and delivery scaffolds must be paired with strategies to prevent cell death to ensure that these cells can survive to form de novo tissue. The Bcl‐2 protein is a prosurvival member of a family of proteins that regulate the mitochondrial pathway of apoptosis. Using several strategies to overexpress the Bcl‐2 protein, we demonstrated a decrease in the mediators of apoptosis in vitro and in vivo. This was shown through the use of two different clinical tissue repair models. Cells overexpressing Bcl‐2 not only survived within the wound environment at a statistically significantly higher rate than control cells, but also increased tissue regeneration. Finally, we used a nonintegrating minicircle technology to achieve this in a potentially clinically applicable strategy for stem cell therapy.