Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane. (22nd August 2013)

• Record Type: Journal Article
• Authors: Qian, Yebin; Zhang, Xian-long; Zeng, Bing-Fang; Jiang, Yao; Shen, Hao; Wang, Qi
• Is Part Of: Connective tissue research. Volume 54:Number 6(2013:Dec.)
• Page Start: 361
• Page End: 366
• Publication Date: 2013-08-22
• Subjects: Cyclooxygenase-2 -- fibroblasts -- RANKL -- substance P -- titanium particles; Connective tissues -- Periodicals; 616.770072
• DOI: 10.3109/03008207.2013.823953
• Languages: English
• ISSNs: 0300-8207

Abstract

Abstract: Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to the failure. Substance P (SP) immunoreactive nerve fibers have been detected in the periprosthetic membrane of aseptic loose hip prostheses. We isolated fibroblasts from periprosthetic membrane. Fibroblasts were examined by real-time RT-PCR and enzyme-linked immunosorbent assay for expression of the receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), cyclooxygenase (COX)-1, and COX-2. Experiments were performed in the presence and absence of titanium particles, SP and NS-398 (a selective COX-2 inhibitor). Titanium particles or SP stimulated RANKL and COX-2 expression in fibroblasts, whereas NS-398 inhibited RANKL production, suggesting a COX-2-mediated event. Moreover, SP enhanced COX-2 and RANKL expression by titanium particles-stimulated fibroblasts. Thus, SP and titanium particles acted synergistically to increase RANKL expression.