Instant Hydrogelation Inspired by Inflammasomes. Issue 26 (24th May 2017)

• Record Type: Journal Article
• Authors: Wang, Huaimin; Feng, Zhaoqianqi; Lu, Alvin; Jiang, Yujie; Wu, Hao; Xu, Bing
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 26(2017)
• Page Start: 7579
• Page End: 7583
• Publication Date: 2017-05-24
• Subjects: hydrogels -- peptides -- self-assembly -- vitamin B6 -- wound healing; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201702783
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Based on the recent near‐atomic structures of the PYRIN domain of ASC in the protein filament of inflammasomes and the observation that the active form of vitamin B6 (pyridoxal phosphate, P5P) modulates the self‐assembly of ASC, we rationally designed an N‐terminal capped nonapeptide (Nap‐FFKKFKLKL, 1 ) to form supramolecular nanofibers consisting of α‐helix. The addition of P5P to the solution of1 results in a hydrogel almost instantly (about 4 seconds). Several other endogenous small molecules (for example, pyridoxal, folinic acid, ATP, and AMP) also convert the solution of1 into a hydrogel. As the demonstration of correlating assemblies of peptides and the relevant protein epitopes, this work illustrates a bioinspired approach to develop supramolecular structures modulated by endogenous small molecules. Abstract : Vitamin B6 and other similar endogenous small molecules induce instant hydrogelation of peptidic epitope from inflammasomes. This is a direct demonstration of correlating supramolecular assemblies of peptides and the relevant protein epitopes for developing instant supramolecular hydrogelation.