Protein‐Templated Fragment Ligations—From Molecular Recognition to Drug Discovery. Issue 26 (31st May 2017)

• Record Type: Journal Article
• Authors: Jaegle, Mike; Wong, Ee Lin; Tauber, Carolin; Nawrotzky, Eric; Arkona, Christoph; Rademann, Jörg
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 26(2017)
• Page Start: 7358
• Page End: 7378
• Publication Date: 2017-05-31
• Subjects: dynamic covalent chemistry -- fragment-based drug discovery -- high-throughput screening -- molecular recognition -- protein-templated reactions; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201610372
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Protein‐templated fragment ligation is a novel concept to support drug discovery and can help to improve the efficacy of protein ligands. Protein‐templated fragment ligations are chemical reactions between small molecules ("fragments") utilizing a protein's surface as a reaction vessel to catalyze the formation of a protein ligand with increased binding affinity. The approach exploits the molecular recognition of reactive small‐molecule fragments by proteins both for ligand assembly and for the identification of bioactive fragment combinations. In this way, chemical synthesis and bioassay are integrated in one single step. This Review discusses the biophysical basis of reversible and irreversible fragment ligations and gives an overview of the available methods to detect protein‐templated ligation products. The chemical scope and recent applications as well as future potential of the concept in drug discovery are reviewed. Abstract : Can drugs be discovered by using proteins as reactors? Proteins have been found to induce reversible and irreversible ligations of protein‐binding fragments. This Review considers the chemistry and the biophysics of such reactions. The potential of template‐catalyzed reactions in drug discovery and as an alternative mode of drug action is discussed.