Combining metabolic evolution and systematic fed-batch optimization for efficient single-cell oil production from sugarcane bagasse. (October 2017)

• Record Type: Journal Article
• Authors: Unrean, Pornkamol; Khajeeram, Sutamat; Champreda, Verawat
• Is Part Of: Renewable energy. Volume 111(2017)
• Page Start: 295
• Page End: 306
• Publication Date: 2017-10
• Subjects: Microbial oils -- Lignocellulosic bioprocess -- Dynamic flux balance -- Model-based fed-batch optimization -- High-titer single cell oil fermentation; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2017.04.018
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: We developed in this study an efficient production process of SCO from sugarcane bagasse hydrolysates using Y. lipolytica . We described a dynamic flux balance model strategy based on a genome-scale metabolic model of Y. lipolytica and SCO fermentation kinetics that applied for rational design of optimized fed-batch process with high single cell oil accumulation. Furthermore, metabolic evolution approach with buoyancy screening was implemented for selecting a high lipid producing yeast strain. The efficient conversion process combined model-based fed-batch process design and evolution-based strain optimization enabling high SCO titer of 45 g/L and 0.25 g-SCO/g-glucose yield from hydrolysates, a 6-fold improvement in titer and 1.4-fold improvement in yield over batch wild-type process. Through this combinatorial optimization effort, we demonstrated the cost-effective, lignocellulose-based SCO process that could meet techno-economic feasibility providing a sustainable alternative to vegetative oils for biofuels and oleochemicals synthesis. Highlights: Dynamic flux balance model was implemented for designing high-SCO-titer bioprocess. An efficient lignocellulosic SCO process was achieved through model-based approach. Metabolic evolution strategy was used for engineering SCO-overproducing strain. Through combinatorial strategies, high SCO accumulation of 45 g/L was accomplished.