Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection. Issue 2 (2nd May 2017)

• Record Type: Journal Article
• Authors: Nadzirah, Sharipah; Hashim, Uda
• Is Part Of: Microelectronics international. Volume 34:Issue 2(2017)
• Page Start: 99
• Page End: 107
• Publication Date: 2017-05-02
• Subjects: Titanium dioxide -- Biosensor -- Interdigitated electrode -- Microelectrodes -- Nanostructure; Microelectronics -- Periodicals; 621.381
• DOI: 10.1108/MI-08-2016-0054
• Languages: English
• ISSNs: 1356-5362

Abstract

Abstract : Purpose: The purpose of this study is to fabricate a transducer-based TiO2 interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for Escherichia. coli O157:H7 DNA detection. Design/methodology/approach: Sol-gel was used to synthesize TiO2 nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter. Findings: TiO2 nanoparticles IDEs with 16-um gap size is able to detect DNA of E. coli in a dry state. Originality/value: This paper describes IDEs for dry-state detection of E. coli O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.