Opioid neurotransmission modulates defensive behavior and fear-induced antinociception in dangerous environments. (23rd June 2017)

• Record Type: Journal Article
• Authors: Coimbra, Norberto Cysne; Calvo, Fabrício; Almada, Rafael Carvalho; Freitas, Renato Leonardo; Paschoalin-Maurin, Tatiana; dos Anjos-Garcia, Tayllon; Elias-Filho, Daoud Hibrahim; Ubiali, Walter Adriano; Lobão-Soares, Bruno; Tracey, Irene
• Is Part Of: Neuroscience. Volume 354(2017)
• Page Start: 178
• Page End: 195
• Publication Date: 2017-06-23
• Subjects: ANOVA analysis of variance -- EAE end-arm exploration -- EPM elevated plus-maze -- ETM elevated T-maze -- FBA flat-back approach -- SAP stretched attend posture; innate fear -- conditioned fear/anticipatory anxiety -- panic attacks -- endogenous opioid peptide-mediated neural system -- prey-versus-rattlesnake pit viper paradigm -- instinctive fear-induced antinociception; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.04.032
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Panic attack-like behavioral responses displayed by Wistar rats threatened by Crotalus durissus terrificus (Reptilia, Viperidae) wild venomous snakes are reverted to fearlessness by the peripheral treatment with naloxone. Background: Photomicrograph of a transverse section of the dorsal midbrain of a Wistar rat showing a fast blue neurotracer-labeled neuron situated in the dorsolateral column of the periaqueductal gray matter, which is rich in opioid peptide neurons and receptors. Highlights: Naloxone causes anxiolytic-like effect and decreases anxiety-induced antinociception in EPM test. Naloxone has no effect on escape/avoidance responses, but decreases fear-induced antinociception in ETM test. Naloxone decreases anxiety- and panic attack-like behaviors in rattlesnake-threatened preys. Naloxone decreases unconditioned fear-induced antinociception in threatened rodents. Abstract: The effects of endogenous opioid peptide antagonists on panic-related responses are controversial. Using elevated mazes and a prey-versus-predator paradigm, we investigated the involvement of the endogenous opioid peptide-mediated system in the modulation of anxiety- and panic attack-induced responses and innate fear-induced antinociception in the present work. Wistar rats were intraperitoneally pretreated with either physiological saline or naloxone at different doses and were subjected to either the elevated plus- or T-maze test or confronted by Crotalus durissus terrificus . The defensive behaviors of the rats were recorded in the presence of the predator and at 24 h after the confrontation, when the animals were placed in the experimental enclosure without the rattlesnake. The peripheral non-specific blockade of opioid receptors had a clear anxiolytic-like effect on the rats subjected to the elevated plus-maze but not on those subjected to the elevated T-maze; however, a clear panicolytic-like effect was observed, i.e., the defensive behaviors decreased, and the prey-versus-predator interaction responses evoked by the presence of the rattlesnakes increased. A similar effect was noted when the rats were exposed to the experimental context in the absence of the venomous snake. After completing all tests, the naloxone-treated groups exhibited less anxiety/fear-induced antinociception than the control group, as measured by the tail-flick test. These findings demonstrate the anxiolytic and panicolytic-like effects of opioid receptor blockade. In addition, the fearlessness behavior displayed by preys treated with naloxone at higher doses enhanced the defensive behavioral responses of venomous snakes.