Native metabotropic glutamate receptor 4 depresses synaptic transmission through an unusual Gαq transduction pathway. (15th July 2017)

• Record Type: Journal Article
• Authors: Chardonnet, Solenne; Bessiron, Thomas; Ramos, Cathy Isaura; Dammak, Raoudha; Richard, Marie-Ange; Boursier, Céline; Cadilhac, Christelle; Coquelle, Frédéric M.; Bossi, Simon; Ango, Fabrice; Le Maréchal, Pierre; Decottignies, Paulette; Berrier, Catherine; McLean, Heather; Daniel, Hervé
• Is Part Of: Neuropharmacology. Volume 121(2017)
• Page Start: 247
• Page End: 260
• Publication Date: 2017-07-15
• Subjects: Presynaptic metabotropic glutamate receptor 4 -- Cerebellar cortex -- Molecular layer interneurons -- Synaptic transmission -- G protein -- Signaling pathway; Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2017.04.036
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: In cerebellar cortex, mGlu4 receptors located on parallel fibers play an essential role in normal motor function, but the molecular mechanisms involved are not yet completely understood. Using a strategy combining biochemical and electrophysiological approaches in the rodent cerebellum, we demonstrate that presynaptic mGlu4 receptors control synaptic transmission through an atypical activation of Gαq proteins. First, the Gαq subunit, PLC and PKC signaling proteins present in cerebellar extracts are retained on affinity chromatography columns grafted with different sequences of the cytoplasmic domain of mGlu4 receptor. The i2 loop and the C terminal domain were used as baits, two domains that are known to play a pivotal role in coupling selectivity and efficacy. Second, in situ proximity ligation assays show that native mGlu4 receptors and Gαq subunits are in close physical proximity in cerebellar cortical slices. Finally, electrophysiological experiments demonstrate that the molecular mechanisms underlying mGlu4 receptor-mediated inhibition of transmitter release at cerebellar Parallel Fiber (PF) – Molecular Layer Interneuron (MLI) synapses involves the Gαq -PLC signaling pathway. Taken together, our results provide compelling evidence that, in the rodent cerebellar cortex, mGlu4 receptors act by coupling to the Gαq protein and PLC effector system to reduce glutamate synaptic transmission. Highlights: Intracellular domains of mGlu4 receptor interact with numerous proteins including Gαq, PLC and PKC. mGlu4 receptor and Gαq are in close proximity in the cerebellar cortex. native mGlu4 receptor reduces glutamatergic transmission at PF – MLI synapses through a Gαq - PLC signaling pathway.