Telomere length profiles in primary human peritoneal mesothelial cells are consistent with senescence. (June 2017)

• Record Type: Journal Article
• Authors: Lopez-Anton, Melisa; Rudolf, András; Baird, Duncan M.; Roger, Laureline; Jones, Rhiannon E.; Witowski, Janusz; Fraser, Donald J.; Bowen, Timothy
• Is Part Of: Mechanisms of ageing and development. Volume 164(2017)
• Page Start: 37
• Page End: 40
• Publication Date: 2017-06
• Subjects: Mesothelium -- Premature senescence -- Single telomere length analysis; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2017.03.010
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Mesothelial cell senescence contributes to malignancy and tissue fibrosis, but the role of telomere erosion remains controversial. Telomere profiles from freshly isolated human peritoneal mesothelial cells and in vitro -aged cells were compared using STELA. STELA revealed heterogeneous telomere lengths from freshly isolated cells consistent with senescence, in vitro aging was telomere-independent. High-resolution mesothelial cell telomere length analysis has the potential to provide clinically useful information. Abstract: Mesothelial cell (MC) senescence contributes to malignancy and tissue fibrosis. The role of telomere erosion in MC senescence remains controversial, with evidence for both telomere-dependent and telomere-independent mechanisms reported. Single telomere length analysis revealed considerable telomere length heterogeneity in freshly isolated human peritoneal MCs, reflecting a heterogeneous proliferative history and providing high-resolution evidence for telomere-dependent senescence. By contrast the attenuated replicative lifespan, lack of telomere erosion and induction of p16 expression in in vitro -aged cells was consistent with stress-induced senescence. Given the potential pathophysiological impact of senescence in mesothelial tissues, high-resolution MC telomere length analysis may provide clinically useful information.