Plasma-derived exosomes contribute to inflammation via the TLR9-NF-κB pathway in chronic heart failure patients. (July 2017)

• Record Type: Journal Article
• Authors: Ye, Wei; Tang, Xiaojun; Yang, Zhengquan; Liu, Chu; Zhang, Xin; Jin, Jing; Lyu, Jianxin
• Is Part Of: Molecular immunology. Volume 87(2017:Jul.)
• Page Start: 114
• Page End: 121
• Publication Date: 2017-07
• Subjects: MtDNA mitochondrial DNA -- TLR9 toll like receptor 9 -- MVBs multivesicular bodies -- ddPCR droplet digital PCR; Exosomes -- Mitochondrial DNA -- Inflammation -- Toll like receptor 9 -- Chronic heart failure; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2017.03.011
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: Plasma-derived exosomes carried mtDNA. Exosomes contribute to inflammation through the mtDNA-TLR9-NF-κB pathway. Chloroquine can block this pathway. Abstract: Exosomes are small vesicles that contain proteins, DNA and RNA, and play an important role in inflammation; however, the underlying mechanism remains unclear. In the present study, we found increased plasma-derived exosomes in chronic heart failure patients compared with healthy controls. Further, our data demonstrated that plasma-derived exosomes carried mtDNA, and triggered an inflammatory response via the TLR9-NF-κB pathway, as well, the inflammatory effect was closely related to exosomal mtDNA copy number. However, the effect could be blocked by chloroquine (CQ), a TLR9 inhibitor. These findings reveal a new mechanism of exosome-induced inflammation, and provide a new perspective for intervention and treatment of inflammation-related diseases, such as chronic heart failure.