COX-2 gene promoter DNA methylation status in eutopic and ectopic endometrium of Egyptian women with endometriosis. (November 2015)

• Record Type: Journal Article
• Authors: Zidan, Haidy E.; Rezk, Noha A.; Alnemr, Amr Abd Almohsen; Abd el Ghany, Amany M.
• Is Part Of: Journal of reproductive immunology. Volume 112(2016)
• Page Start: 63
• Page End: 67
• Publication Date: 2015-11
• Subjects: COX-2 cyclooxygenase-2 -- PGs prostaglandins -- MS-PCR methylation specific polymerase chain reaction -- NF-kB nuclear factor-Kappa beta -- NF-IL6 nuclear factor for interleukin-6 expression -- CRE cyclic AMP response element; Endometriosis -- DNA hypomethylation -- COX-2 mRNA expression -- Epigenetics; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2015.06.093
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: Significantly higher levels of COX-2 were found in ectopic endometriotic tissue than in controls. The frequencies of methylation status of the NF-IL6 site within the COX-2 promoter were significantly decreased in the eutopic groups. There were higher levels of COX-2 in ectopic endometriotic tissue compared with eutopic tissue. Abstract: The pathophysiology of COX-2 expression in endometriosis is a matter of debate. The aim was to investigate the role of DNA methylation of the NF-IL6 site within the promoter of COX-2 gene in the pathogenesis of endometriosis. The endometrial tissues (ectopic and eutopic) were collected from 60 women with endometriosis and 30 women without endometriosis (control group). The methylation status of COX-2 was examined by methylation-specific PCR. Quantitative real-time PCR (RT-PCR) was performed to measure COX-2 mRNA levels in endometrial tissues. We found significantly higher levels of COX-2 in ectopic endometriotic tissue compared with eutopic tissue. Also, we found that the frequencies of methylation status of the NF-IL6 site within the COX-2 promoter in the eutopic and ectopic endometrial tissues of endometriosis groups were significantly decreased in comparison to controls ( P = 0.002, P = 0.000 respectively). Our study demonstrated that DNA hypomethylation of the NF-IL6 site within the promoter of COX-2 gene could be a key mechanism for its elevated expression in the eutopic and ectopic tissues of endometriosis.