Exosomes: Ultrastructural evidence in epithelial cells of Malpighian tubules. (September 2017)

• Record Type: Journal Article
• Authors: Giglio, Anita; Perrotta, Ida Daniela; Brandmayr, Pietro
• Is Part Of: Micron. Volume 100(2017)
• Page Start: 34
• Page End: 37
• Publication Date: 2017-09
• Subjects: Exosomes -- Multivesicular bodies -- Endosomes -- Carabid beetles; Microscopy -- Periodicals; Electron Probe Microanalysis -- Periodicals; Microscopy -- Periodicals; Microscopie -- Périodiques; Microscopy; Periodicals; 502.82
• DOI: 10.1016/j.micron.2017.04.008
• Languages: English
• ISSNs: 0968-4328

Abstract

Highlights: Exosomes are small vesicles released by most cell types and are involved in cell communication. Intraluminal vesicles influence intercellular trafficking in cell of Malpighian tubules. Physiological and pathological processes in insect cells can involve exosomes. Abstract: Exosomes in Malpighian tubules of Calathus fuscipes (Coleoptera: Carabidae) were investigated under transmission electron microscopy. Ultrastructural analyses showed a wide number of multivesicular bodies localized in the apical portion of epithelial cells. Each multivesicular body encloses from 15 to 80 intraluminal vesicles (about 50 nm in diameter), originating through inward budding of late endosomes that package molecules into luminal membrane-bound structures. Subsequently they are released as exosomes through exocytosis of multivesicular bodies into the extracellular space after fusion with plasma membrane. Our results are the base for further investigation on the role of exosomes in functional polarization of tubule cells and on cell-to-cell communication in insects.