Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages. (5th August 2017)

• Record Type: Journal Article
• Authors: Kudryavtseva, Valeriya; Stankevich, Ksenia; Gudima, Alexandu; Kibler, Elina; Zhukov, Yuri; Bolbasov, Evgeniy; Malashicheva, Anna; Zhuravlev, Mikhail; Riabov, Vladimir; Liu, Tengfei; Filimonov, Victor; Remnev, Gennady; Klüter, Harald; Kzhyshkowska, Julia; Tverdokhlebov, Sergei
• Is Part Of: Materials & design. Volume 127(2017)
• Page Start: 261
• Page End: 271
• Publication Date: 2017-08-05
• Subjects: Polylactic acid (PLA) -- Hyaluronic acid (HA) -- Tissue engineering scaffold (TES) -- Plasma -- Macrophages -- Cytokines; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2017.04.079
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface. Graphical abstract: Highlights: Polylactic acid based scaffolds with improved long-term hydrophilicity were produced. Donor-specific reaction of macrophages to the modified polylactic acid based scaffolds was observed. Polylactic acid based scaffolds coated with hyaluronic acid have enhanced biocompatibility and pro-angiogenic activity.