Interferon-induced protein 56 (IFI56) is induced by VHSV infection but not by bacterial infection in olive flounder (Paralichthys olivaceus). Issue 66 (July 2017)

• Record Type: Journal Article
• Authors: Hwang, Jee Youn; Ahn, Sang Jung; Kwon, Mun-Gyeong; Seo, Jung Soo; Hwang, Seong Don; Son, Maeng-Hyun
• Is Part Of: Fish & shellfish immunology. Issue 66(2017)
• Page Start: 382
• Page End: 389
• Publication Date: 2017-07
• Subjects: Interferon-induced protein 56 -- Viral hemorrhagic septicemia virus -- Olive flounder -- Interferon -- Tissue expression; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.05.027
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Interferon-inducible protein 56 ( IFI56, also known as ISG56/ IFIT1, interferon-induced protein with tetratricopeptide repeats 1) is strongly induced in response to interferon and a potent inhibitor of viral replication and translational initiation. Here, we describe the identification of IFI56 ( OfIFI56 ) in olive flounder, its characteristic features, and expression levels in various tissues before and after viral hemorrhagic septicemia virus (VHSV) infection. The full-length OfIFI56 sequence was identified from rapid amplification of cDNA ends PCR. The complete coding sequence of OfIFI56 is 1971 bp in length and encodes 431 amino acids. The putative OfIFI56 protein has multiple tetratricopeptide (TPR) motifs, which regulate diverse biological processes, such as organelle targeting, protein import, and vesicle fusion. Based on sequence analysis, the Larimichthys crocea IFI56 protein (61%) had the highest sequence homology to OfIFI56. In healthy olive flounder, OfIFI56 mRNA expression was detected in many tissues such as intestine, gill, head kidney, heart, spleen, and trunk kidney tissues. After VHSV challenge, OfIFI56 mRNA was significantly up-regulated in these tissues. Additionally, OfIFI56 expression was induced by poly I:C but not by Streptococcus parauberis and S. iniae infection or lipopolysaccharide injection in kidney and spleen tissues of olive flounder. These results demonstrate that piscine OfIFI56 expression is not induced by bacterial infection but is selectively induced by viral infection, especially VHSV, and that OfIFI56 may play an important role in the host response against VHSV infection. Highlights: The fish IFI56/IFIT1 family including OfIFI56 grouped with IFI56 and not fish IFI58. OfIFI56 mRNA was up-regulated in all tissues except muscle after VHS virus challenge. OfIFI56 expression was not induced by Streptococcus parauberis and S. iniae infection and LPS injection in olive flounder. OfIFI56 mRNA expression markedly increased in all tissues after poly I: C infection.