Organic dye doped nanoparticles with NIR emission and biocompatibility for ultra-deep in vivo two-photon microscopy under 1040 nm femtosecond excitation. (August 2017)

• Record Type: Journal Article
• Authors: Alifu, Nuernisha; Yan, Lulin; Zhang, Hequn; Zebibula, Abudureheman; Zhu, Zhenggang; Xi, Wang; Roe, Anna Wang; Xu, Bin; Tian, Wenjing; Qian, Jun
• Is Part Of: Dyes and pigments. Volume 143(2017)
• Page Start: 76
• Page End: 85
• Publication Date: 2017-08
• Subjects: Organic dye nanoparticles -- NIR fluorescence -- Two-photon fluorescence -- In vivo -- Brain imaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2017.04.017
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Nanoparticle-assisted near-infrared (NIR) bioimaging and two-photon fluorescence microscopy (TPFM) are two important technologies in biophotonic research. In this work, we synthesize the dye named 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TPABDFN), which had a large two-photon absorption cross-section and bright NIR emission. The dyes were then encapsulated with poly(styrene- co -maleic anhydride) (PSMA), forming fluorescent nanoparticles. The TPABDFN-PSMA nanoparticles possessed high chemical and optical stability, good biocompatibility, as well as large two-photon absorption cross-section (5.56 × 10 5 GM). Furthermore, we combined NIR bioimaging and TPFM together, and utilized TPABDFN-PSMA nanoparticles as fluorescent contrast agents for two-photon excited NIR microscopic imaging, with a 1040 nm-femtosecond laser. In vivo angiography of mice ear and brain was performed. Due to the deep penetration capability of both 1040 nm-excitation and NIR emission light, a very large in vivo microscopic imaging depth (∼1.2 mm) was achieved. NIR emissive and biocompatible TPABDFN-PSMA nanoparticles have great potential in disease diagnosis and clinical therapies, where deep-tissue imaging is required. Graphical abstract: NIR emissive TPABDFN-PSMA-PEG nanoparticles with large two-photon absorption cross-section were intravenously injected into a mouse, and three-dimensional architecture of the blood vessels in the mouse brain was reconstructed vividly with two-photon fluorescence microscopy at imaging depth ∼1200 μm. Highlights: NIR emissive organic dye TPABDFN with well nonlinear coefficient were synthesized. TPABDFN-PSMA NPs show excellent optical, chemical stability and biocompatibility. TPABDFN-PSMA NPs were found large two-photon absorption cross-section at 1040 nm. 3D in vivo brain vascular imaging of mice was reconstructed at 1040 nm-fs by NPs. 1.2 mm imaging depth was achieved in vivo brain vascular imaging of mice at 1040 nm.