Assessment of advanced glycated end product accumulation in skin using auto fluorescence multispectral imaging. (1st June 2017)

• Record Type: Journal Article
• Authors: Larsson, Marcus; Favilla, Riccardo; Strömberg, Tomas
• Is Part Of: Computers in biology and medicine. Volume 85(2017)
• Page Start: 106
• Page End: 111
• Publication Date: 2017-06-01
• Subjects: Advanced glycation end products -- Multispectral imaging -- Noninvasive -- Auto fluorescence -- Self-monitoring; Medicine -- Data processing -- Periodicals; Biology -- Data processing -- Periodicals; 610.285
• DOI: 10.1016/j.compbiomed.2016.04.005
• Languages: English
• ISSNs: 0010-4825

Abstract

Abstract: Several studies have shown that advanced glycation end products (AGE) play a role in both the microvascular and macrovascular complications of diabetes and are closely linked to inflammation and atherosclerosis. AGEs accumulate in skin and can be detected using their auto fluorescence (AF). A significant correlation exists between AGE AF and the levels of AGEs as obtained from skin biopsies. A commercial device, the AGE Reader, has become available to assess skin AF for clinical purposes but, while displaying promising results, it is limited to single-point measurements performed in contact to skin tissue. Furthermore, in vivo imaging of AGE accumulation is virtually unexplored. We proposed a non-invasive, contact-less novel technique for quantifying fluorescent AGE deposits in skin tissue using a multispectral imaging camera setup (MSI) during ultraviolet (UV) exposure. Imaging involved applying a region-of-interest mask, avoiding specular reflections and a simple calibration. Results of a study conducted on 16 subjects with skin types ranging from fair to deeply pigmented skin, showed that AGE measured with MSI in forearm skin was significantly correlated with the AGE reference method (AGE Reader on forearm skin, R =0.68, p =0.005). AGE measured in facial skin was borderline significantly related to AGE Reader on forearm skin ( R =0.47, p =0.078). These results support the use of the technique in devices for non-touch measurement of AGE content in either facial or forearm skin tissue over time. Graphical abstract: Highlights: Advanced glycated end products (AGE) are imaged with a two camera setup. A UV LED light source at 365 nm is used for inducing AGE auto fluorescence (AF). Skin tissue AGE is assessed as the relative amount of AF in the 475 nm region. Modulated light is used for ambient light cancelation. A significant correlation to current single-point systems shows proof-of-principle.