Glutathione biosynthesis plays an important role against 4-tert-octylphenol-induced oxidative stress in Ceratophyllum demersum. (September 2017)

• Record Type: Journal Article
• Authors: Cahyanurani, Annisa' Bias; Chiu, Kuo-Hsun; Wu, Tsung-Meng
• Is Part Of: Chemosphere. Volume 183(2017)
• Page Start: 565
• Page End: 573
• Publication Date: 2017-09
• Subjects: 4-tert-Octylphenol -- Ceratophyllum demersum -- Oxidative stress -- ROS -- GSH; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.05.150
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: 4- tert -octylphenol (OP) is a persistent environmental pollutant with an endocrine-disrupting property. In the present study, we examined the effect of various concentrations of OP (0, 0.5, 1, 1.5, 2 and 3 mg L −1 ) applied to an aquatic plant, the submersed macrophyte Ceratophyllum demersum . The toxic effect caused by OP inhibited the plant's growth rate, reduced total chlorophyll content and increased levels of the reactive oxygen species (ROS) O2 − and H2 O2 . OP treatment significantly increased the activities of antioxidant enzymes including superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase. The contents of the non-enzymatic antioxidant glutathione (GSH) and ratio of GSH to glutathione disulfide were markedly increased with OP treatment. Pretreatment with buthionine sulfoximine, a specific and potent inhibitor of GSH biosynthesis, significantly reduced total GSH content and conferred a more severe toxic phenotype on OP exposure. Thus, with OP-induced oxidative stress, C. demersum might actively regulate the antioxidant machinery, especially the biosynthesis and redox state of GSH. Highlights: The toxic effects of octylphenol (OP) on Ceratophyllum demersum have been studied. OP caused oxidative stress in C. demersum . Glutathione (GSH) contents and GSH/GSSG were markedly increased under OP exposure. Reduction of GSH by BSO caused severe toxic phenotype under OP exposure.