Occurrence of marine algal toxins in oyster and phytoplankton samples in Daya Bay, South China Sea. (September 2017)

• Record Type: Journal Article
• Authors: Jiang, Tao; Liu, Lei; Li, Yang; Zhang, Jing; Tan, Zhijun; Wu, Haiyan; Jiang, Tianjiu; Lu, Songhui
• Is Part Of: Chemosphere. Volume 183(2017)
• Page Start: 80
• Page End: 88
• Publication Date: 2017-09
• Subjects: Shellfish toxins -- Okadaic acid -- Pectenotoxin -- Dinophysis -- Domoic acid -- Pseudo-nitzschia; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.05.067
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The occurrence and seasonal variations of marine algal toxins in phytoplankton and oyster samples in Daya Bay (DYB), South China Sea were investigated. Two Dinophysis species, namely, D. caudata and D. acuminata complex, were identified as Okadaic acid (OA)/pectenotoxin (PTX) related species. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis demonstrated that 2.04–14.47 pg PTX2 per cell was the predominant toxin in single-cell isolates of D. caudata . D. acuminata was not subjected to toxin analysis. The occurrence of OAs in phytoplankton concentrates of net-haul sample coincided with the presence of D. accuminata complex, suggesting that this species is most likely an OA producer in this sea area. OA, dinophysistoxins-1 (DTX1), PTX2, PTX2sa, gymnodimine (GYM), homoyessotoxin (homoYTX), and domoic acid (DA) demonstrated positive results in net haul samples. To our best knowledge, this paper is the first to report the detection of GYM, DA, and homoYTX in phytoplankton samples in Chinese coastal waters. Among the algal toxins, GYM demonstrated the highest frequency of positive detections in phytoplankton concentrates (13/17). Five compounds of algal toxins, including OA, DTX1, PTX2, PTX2sa, and GYM, were detected in oyster samples. DA and homoYTX were not detected in oysters despite of positive detections for both in the phytoplankton concentrates. However, neither the presence nor absence of DA in oysters can be determined because extraction conditions with 100% methanol used to isolate toxins from oysters (recommended by the EU-Harmonised Standard Operating Procedure, 2015) would likely be unsuitable for this water-soluble toxin. In addition, transformation of DA during the digestion process of oysters may also be involved in the negative detections of this toxin. GYM exhibited the highest frequency of positive results in oysters (14/17). OAs were only detected in the hydrolyzed oyster samples. The detection rates of PTX and PTX2sa in oysters were lower than those in the net haul samples. Highlights: D. caudata and D. acuminata complex were identified as Okadaic acid (OA)/pectenotoxin (PTX) related species. PTX2 was the predominant toxin in D. caudata, while OAs were related with D. acuminata complex. This paper is the first to report the detection of GYM, DA, and homoYTX in phytoplankton samples in Chinese coastal waters. GYM exhibited the highest frequency of positive detections in phytoplankton concentrates (13/17) and oysters (14/17).