Cloning and functional characterization of a retinoid X receptor orthologue in Platynereis dumerilii: An evolutionary and toxicological perspective. (September 2017)

• Record Type: Journal Article
• Authors: André, Ana; Ruivo, Raquel; Capitão, Ana; Froufe, Elsa; Páscoa, Inês; Costa Castro, Luís Filipe; Santos, Miguel Machado
• Is Part Of: Chemosphere. Volume 182(2017)
• Page Start: 753
• Page End: 761
• Publication Date: 2017-09
• Subjects: Retinoid X receptor -- Annelid -- Endocrine disruption -- Evolution -- Tributyltin -- Triphenyltin; Bp Base pairs -- COI Cytochrome oxidase I -- DBD DNA binding domain -- EDCs Endocrine disrupting chemicals -- LBD Ligand binding domain -- LBP Ligand binding pocket -- NRs Nuclear receptors -- ORF Open reading frame -- PCR Polymerase chain reaction -- PPAR Peroxisome proliferator-activated receptor -- RA All-trans-retinoic acid -- RACE Rapid amplification of cDNA ends -- RAR Retinoid acid receptor -- RXR Retinoid X receptors -- TBT Tributyltin -- TPT Triphenyltin -- TR Thyroid hormone receptor -- USP Ultraspiracle -- 9-Cis-RA 9-cis-retinoic acid; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.05.064
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In the present work we provide the first isolation and functional characterization of a RXR orthologue in an annelid species, the Platynereis dumerilii . Using an in vitro luciferase reporter assay we evaluated the annelid receptor ability to respond to ligand 9- cis -retinoic acid, TBT and TPT. Our results show that the annelid RXR responds to 9- cis -retinoic acid and to the organotins by activating reporter gene transcription. The findings suggest a conserved mode of action of the receptor in response to a common signaling molecule and modulation by organotin compounds between vertebrates and Lophotrochozoans. Graphical abstract: Highlights: First time isolation and functional characterization of RXR from Annelids. Annelid RXR conserved transcriptional activation response in the presence of 9-cis-RA. Annelids RXR-signaling pathway is a target of environmental pollutants including organotins.