Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis. (15th September 2017)

• Record Type: Journal Article
• Authors: Zou, Yuan-Feng; Fu, Yu-Ping; Chen, Xing-Fu; Austarheim, Ingvild; Inngjerdingen, Kari Tvete; Huang, Chao; Lei, Fei-Yi; Song, Xu; Li, Lixia; Ye, Gang; Eticha, Lemlem Dugassa; Yin, Zhongqiong; Paulsen, Berit Smestad
• Is Part Of: Carbohydrate polymers. Volume 172(2017)
• Page Start: 306
• Page End: 314
• Publication Date: 2017-09-15
• Subjects: d-Mannose (PubChem CID: 18950) -- l-Rhamnose (PubChem CID: 25310) -- d-Glucuronic acid (PubChem CID: 94715) -- d-Galactose (PubChem CID: 6036) -- d-Glucose (PubChem CID: 5793) -- d-Galacturonic acid (PubChem CID: 439215) -- d-Xylose (PubChem CID: 135191) -- l-Arabinose (PubChem CID: 439195) -- d-Fructose (PubChem CID: 5984) -- Trifluoroacetic acid (PubChem CID: 6422); Gentiana crassicaulis -- Polysaccharides -- Purification -- Complement system; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.04.049
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Two polysaccharide fractions were purified from roots of Gentiana crassicaulis . These two fractions were pectic polysaccharides. These two polysaccharides could be used as a potential natural immunomodulator. Abstract: Two polysaccharides, GCP-I-I and GCP-II-I, were obtained from 100 °C water extracts of Gentiana crassicaulis roots by DEAE anion exchange chromatography and gel filtration. The results from methanolysis, methylation, FT-IR and NMR, indicated that these two fractions are typical pectic polysaccharides, with HG and RG-I regions and AG-I/AG-II side chains, and some of the galacturonic acid units of fraction GCP-I-I were methyl esterified. Fractions GCP-I-I and GCP-II-I, both exhibited potent complement fixation, and fraction GCP-I-I was more potent than positive control BPII. The higher complement fixation activity obtained in fraction GCP-I-I may be due to the higher Mw and/or higher amount of AG-II present in fraction GCP-I-I than fraction GCP-II-I. The polysaccharides from G. crassicaulis could be used as a potential natural immunomodulator.