Investigating the potential of Oxymatrine as a psoriasis therapy. (1st June 2017)

• Record Type: Journal Article
• Authors: Chen, Qian; Zhou, Hui; Yang, Yinxue; Chi, Mingwei; Xie, Nan; Zhang, Hong; Deng, Xingwang; Leavesley, David; Shi, Huijuan; Xie, Yan
• Is Part Of: Chemico-biological interactions. Volume 271(2017)
• Page Start: 59
• Page End: 66
• Publication Date: 2017-06-01
• Subjects: Psoriasis -- Oxymatrine -- Hyperproliferation -- Keratinocytes; OMT Oxymatrine -- HK human skin keratinocytes -- UVB Ultraviolet B -- PASI Psoriasis Area Severity Index -- SFM serum-free media -- FG Full Green's medium -- BrdU Bromodeoxyuridine -- 3-D three-dimensional -- DED-HSE de-epidermised dermis human skin equivalent -- AE1-AE3 Pan-Cytokeratin antibody -- 2-D two-dimensional; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2017.04.020
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Psoriasis vulgaris is a chronic inflammatory skin disease, stubbornly intractable, with substantial consequences for patient physical and mental welfare. Approaches currently available to treat psoriasis are not satisfactory due to undesirable side-effects or expense. Psoriasis is characterized by hyperproliferation and inflammation. Oxymatrine, an active component extracted from Sophora flavescens, has been demonstrated to possess anti-proliferation, anti-inflammatory, anti-tumorigenic, immune regulation and pro-apoptotic properties. This investigation presents a detailed retrospective review examining the effect of Oxymatrine on psoriasis and investigates the mechanisms underlying patient responses to Oxymatrine. We confirm that Oxymatrine administration significantly reduced the Psoriasis Area Severity Index score, with high efficacy compared to the control group. In addition, we have found that Oxymatrine significantly inhibits the viability, proliferation and differentiation of human keratinocyte in vitro. Immunohistochemical analysis indicates Oxymatrine significantly suppresses the expression of Pan-Cytokeratin, p63 and keratin 10. The results indicate that the suppression of p63 expression may lead to the anti-proliferation effect of Oxymatrine on human skin keratinocytes. Oxymatrine does not affect the formation of basement membrane, which is very important to maintain the normal function of human skin keratinocytes. In summary, Oxymatrine offers an effective, economical, and safe treatment for patients presenting with intractable psoriasis vulgaris. Highlights: Oxymatrine induces marked improvements in psoriasis vulgaris symptoms. Oxymatrine inhibits the viability and proliferation of keratinocytes. Oxymatrine suppresses the expression of AE1-AE3, p63 and keratin 10. Oxymatrine does not affect the formation of basement membrane.