Steam-exploded biomass saccharification is predominately affected by lignocellulose porosity and largely enhanced by Tween-80 in Miscanthus. (September 2017)

• Record Type: Journal Article
• Authors: Sun, Dan; Alam, Aftab; Tu, Yuanyuan; Zhou, Shiguang; Wang, Yanting; Xia, Tao; Huang, Jiangfeng; Li, Ying; Zahoor, ; Wei, Xiaoyang; Hao, Bo; Peng, Liangcai
• Is Part Of: Bioresource technology. Volume 239(2017)
• Page Start: 74
• Page End: 81
• Publication Date: 2017-09
• Subjects: Miscanthus -- Lignocellulose features -- Biomass porosity -- Steam explosion -- Biomass saccharification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.04.114
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Diverse cell wall compositions in ten representative Miscanthus accessions. Largely varied biomass enzymatic saccharification in the stem-exploded (SE) residues. Supply with 2% Tween-80 led to much enhanced SE biomass enzymatic digestibility. Four wall polymer features negatively affected SE biomass saccharification. Lignocellulose porosity was the unique positive factor on SE enzymatic hydrolysis. Abstract: In this study, total ten Miscanthus accessions exhibited diverse cell wall compositions, leading to largely varied hexoses yields at 17%–40% (% cellulose) released from direct enzymatic hydrolysis of steam-exploded (SE) residues. Further supplied with 2% Tween-80 into the enzymatic digestion, the Mis7 accession showed the higher hexose yield by 14.8-fold than that of raw material, whereas the Mis10 had the highest hexoses yield at 77% among ten Miscanthus accessions. Significantly, this study identified four wall polymer features that negatively affect biomass saccharification as p < 0.05 or 0.01 in the SE residues, including cellulose DP, Xyl and Ara of hemicellulose, and S-monomer of lignin. Based on Simons' stain, the SE porosity (defined by DY/DB) was examined to be the unique positive factor on biomass enzymatic digestion. Hence, this study provides the potential strategy to enhance biomass saccharification using optimal biomass process technology and related genetic breeding in Miscanthus and beyond.