Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood. Issue 1 (7th February 2017)

• Record Type: Journal Article
• Authors: Hamilton, Carly A.; Mahan, Suman; Bell, Charlotte R.; Villarreal‐Ramos, Bernardo; Charleston, Bryan; Entrican, Gary; Hope, Jayne C.
• Is Part Of: Immunology. Volume 151:Issue 1(2017)
• Page Start: 89
• Page End: 97
• Publication Date: 2017-02-07
• Subjects: natural killer cells -- recirculation; Immunology -- Periodicals
• DOI: 10.1111/imm.12708
• Languages: English
• ISSNs: 0019-2805

Abstract

Summary: Natural killer (NK) cells are widely distributed in lymphoid and non‐lymphoid tissues, but little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady‐state and also for determining the roles for NK cells in vaccine‐induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady‐state conditions was investigated in cattle using the pseudo‐afferent lymphatic cannulation model. CD2 + CD25 lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2 − CD25 hi NK cells were the main subset present within the skin‐draining afferent lymphatic vessels and lymph nodes, indicating that CD2 − NK cells are the principal NK cell subset trafficking to lymph nodes via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph, which were predominantly of the CD2 − subset, indicating that NK cells can egress from lymph nodes and return to circulation in steady‐state conditions. These compartmentalization data indicate that NK cells represent a population of recirculating lymphocytes in steady‐state conditions and therefore may be important during immune responses to vaccination or infection. Abstract : Through the use of afferent and efferent lymphatic cannulation models, this manuscript demonstrates that NK cells are present in the blood, afferent lymph and lymph node of calves in steady‐state conditions. Furthermore, NK cells were also found in efferent lymph therefore indicating that NK cells can egress from the lymph node and return to circulation. Thus, bovine NK cells may represent a population of recirculating lymphocytes and play a role in immune surveillance.