Origin of α-mannosidase activity in CSF. (June 2017)

• Record Type: Journal Article
• Authors: Tasegian, Anna; Paciotti, Silvia; Ceccarini, Maria Rachele; Codini, Michela; Moors, Tim; Chiasserini, Davide; Albi, Elisabetta; Winchester, Bryan; van de Berg, Wilma D.J.; Parnetti, Lucilla; Beccari, Tommaso
• Is Part Of: International journal of biochemistry & cell biology. Volume 87(2017)
• Page Start: 34
• Page End: 37
• Publication Date: 2017-06
• Subjects: AD Alzheimer's disease -- BBB blood–brain barrier -- CSF cerebrospinal fluid -- DLB dementia with Lewy bodies -- ERT enzyme replacement therapy -- EV extracellular vesicles -- FTD Fronto-Temporal Dementia -- LP lumbar puncture -- MAN2B1 lysosomal α-mannosidase -- MAN2C1 cytosolic α-mannosidase -- M6P mannose-6-phosphate -- M6PR mannose-6-phosphate receptors -- ONDs other minor neurological disorders -- PD Parkinson's disease; Lysosomes -- CSF -- α-Mannosidase -- MAN2B1 -- Brain -- Parkinson; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2017.03.016
• Languages: English
• ISSNs: 1357-2725

Abstract

Abstract: The α-mannosidase activity in human frontal gyrus, cerebrospinal fluid and plasma has been analyzed by DEAE-cellulose chromatography to investigate the origin of the α-mannosidase activity in cerebrospinal fluid (CSF). The profile of α-mannosidase isoenzymes obtained in CSF was similar to that in the frontal gyrus but different from that in human plasma. In particular the two characteristic peaks of lysosomal α-mannosidase, A and B, which have a pH-optimum of 4.5 and are found in human tissues, were present in both the frontal gyrus and CSF. In contrast the majority of α-mannosidase activity in human plasma was due to the so called intermediate form, which has a pH-optimum of 5.5. The results suggest that the intermediate form of α-mannosidase in plasma does not cross the blood–brain barrier and that the α-mannosidase activity present in the cerebrospinal fluid is of lysosomal type and of brain origin. Thus the α-mannosidase activity in cerebrospinal fluid might mirror the brain pathological changes linked to neurodegenerative disorders such as Parkinson's disease.