Derivatives of Dapsone (dap): Synthesis and Study on In Vitro Anticancer Activity and DNA Laddering Against Hep G2 and C6 Human Cancer Cell Lines. Issue 16 (1st June 2017)

• Record Type: Journal Article
• Authors: Pillai, Vineeta; Kadu, Rahul; Buch, Lipi; Singh, Vinay K.
• Is Part Of: ChemistrySelect. Volume 2:Issue 16(2017)
• Page Start: 4382
• Page End: 4391
• Publication Date: 2017-06-01
• Subjects: Anticancer -- Density-functional theory -- Dithiocarbamate -- Metallomacrocyclic -- Sulfone; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201700701
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Interesting biological profile of dapsone (dap) has encouraged us to derivatize it further into a novel series of diamines 4, 4'‐bis(2‐(alkylamino) acetamido) diphenylsulfoneL 1 ‐L 3 and their ensuing metallomacrocyclic complexes of the type [M2 ‐ μ 2 ‐bis‐{( κ 2 S, S ‐S2 CN(R)CH2 CONHC6 H4 )2 SO2 }] {R= Cy, M=Ni II 1 a, Cu II 1 b, Zn II 1 c ; R= i Pr, M=Ni II 2 a, Cu II 2 b, Zn II 2 c ; R= n Bu, M=Ni II 3 a, Cu II 3 b, Zn II 3 c }. These compounds were characterized by standard spectroscopic methods. A DFT level calculation has been performed on selected compounds. In vitro anticancer activity against Hep G2 (hepatoma) and C6 (Glioblastoma) cell lines suggests specificity of these compounds for cancer cells over normal liver cells. Interestingly, complex 2c holding zinc(II) and N ‐ i Pr substituents shows nearly 3 fold better cytotoxic activity against both Hep G2 (8.47±0.016 μg/mL) and C6 (4.3±0.019 μg/mL) cell lines, compared to the reference drug Cisplatin. The morphological changes and moderate to heavy DNA laddering clearly demonstrate the induction of apoptotic cell death, required for major chemical therapeutic implications. Abstract : Dapsone was selected as lead compound to derivatize into a number of well characterized organic and coordination compounds. The specificity of these compounds for cancer cells over normal liver cells was revealed by their in vitro anticancer activity against Hep G2 and C6 cell lines. Morphological changes and DNA laddering clearly supports the induction of apoptotic cell death.