Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics. (24th January 2017)

• Record Type: Journal Article
• Authors: Hu, Qingping; Wei, Jianteng; Liu, Yewei; Fei, Xiulan; Hao, Yuwei; Pei, Dong; Di, Duolong
• Is Part Of: Biomedical chromatography. Volume 31:Number 7(2017:Jul.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-01-24
• Subjects: alcohol‐induced oxidative stress -- biomarkers -- human liver L‐02 cells -- orthogonal partial least‐squares discriminate analysis -- principal components analysis; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3907
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Biomarkers involved in alcohol‐induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol‐induced oxidative stress in human liver L‐02 cells was used to discover the potential biomarkers. Metabolites from L‐02 cells induced by alcohol were measured by high‐performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least‐squares discriminate analysis). Based on the retention time, UV spectrum and LC–MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol‐induced oxidative stress, namely, malic acid, oxidized glutathione, γ‐glutamyl‐cysteinyl‐glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches.