Decreased expression of MMP-9 in CD8+ cells in placenta with severe preeclampsia. (19th May 2017)

• Record Type: Journal Article
• Authors: Orlovic, M; Tomic, V; Vukojevic, K; Hudic, I; Mandic, V; Azinovic, I; Soldo, D; Kajic, M; Soljic, V
• Is Part Of: Biotechnic & histochemistry. Volume 92:Number 4(2017)
• Page Start: 288
• Page End: 296
• Publication Date: 2017-05-19
• Subjects: CD4 -- CD8 -- decidua basalis -- immunohistochemistry -- MMP-9 -- placenta -- preeclampsia; Histochemistry -- Periodicals; Stains and staining (Microscopy) -- Periodicals; Biotechnology -- Periodicals; 572
• DOI: 10.1080/10520295.2017.1309069
• Languages: English
• ISSNs: 1052-0295

Abstract

Abstract: We compared the number of CD4-positive (CD4 + ) and CD8-positive (CD8 + ) cells in severe and non-severe preeclampsia (PE), and in normal pregnancy. We also evaluated the expression of matrix metalloproteinase 9 (MMP-9) in CD4 + and CD8 + cells. Immunohistochemistry for CD4 + and CD8 + was performed on the decidua basalis of 15 severe and 13 non-severe PE women and compared to decidual tissue of 19 normal pregnancies (control group). Co-expression of MMP-9 with CD8 + and CD4 + cells was determined by double immunofluorescence staining. The median number of CD8 + cells/mm 2 was significantly lower for the severe PE group than for the normal pregnancy group, as was the number of CD4 + cells and MMP-9 + CD8 + cells. No statistical difference was found between the non-severe PE group and the normal pregnancy group. The significant decrease of CD4 +, CD8 + and MMP-9 + CD8 + cells at the fetal-maternal interface only in the severe PE group suggests that immunological disorders play a role in the pathophysiology of severe PE.