Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates. Issue 20 (16th May 2017)

• Record Type: Journal Article
• Authors: Wu, Kefeng; Ma, Changbei; Liu, Haisheng; He, Hailun; Zeng, Weimin; Wang, Kemin
• Is Part Of: Analytical methods. Volume 9:Issue 20(2017)
• Page Start: 3055
• Page End: 3060
• Publication Date: 2017-05-16
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c7ay00709d
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A simple fluorescence based biosensor for label-free detection of RNase H activity based on Tb 3+ -induced G-quadruplex conjugates is reported. Abstract : Ribonuclease H (RNase H), a highly conserved damage-repair protein, hydrolyzes RNA in the DNA:RNA hybrid duplex and breaks RNA/DNA junctions. In this study, we demonstrated a unique Tb 3+ -based fluorescence assay that is low cost, facile, and label-free for assaying RNase H activity and inhibitions by using a G-quadruplex formation strategy. This novel assay method can detect RNase H at the lowest detection limit of 2 U mL −1 under optimal conditions. We demonstrated the utility of the assay by antibiotics screening and identified ethidium bromide (EB) and Gentamycin as RNase H inhibitors. This approach demonstrated that Tb 3+ could be used as a functional tool in specific fields in the future.