Digitally encoded silica microparticles for multiplexed nucleic acid detection. Issue 43 (16th May 2017)

• Record Type: Journal Article
• Authors: Xu, Weiwei; Chen, Chao; Ma, Xiaodong; Yuan, Lihua; Liu, Shenquan; Zheng, Kexiao; Li, Jiong
• Is Part Of: Chemical communications. Volume 53:Issue 43(2017)
• Page Start: 5866
• Page End: 5869
• Publication Date: 2017-05-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc01974b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : By integrating a digitally encoded suspension array with a novel multiplex nested asymmetric PCR, an efficient strategy was developed for HPV genotyping. Abstract : A robust high throughput suspension array was proposed to analyze biomolecules. To exploit its capacity for nucleic acid detection, we designed a multiplex nested asymmetric PCR (MNAS PCR) that can produce single stranded DNA (ssDNA) efficiently without complicated optimization. Multiplexed HPV genotyping was demonstrated with high selectivity, high sensitivity and rapid hybridization within 20 minutes.